Response of Infusorian Cells to Injury Caused by a Laser Microbeam
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چکیده
The effects of pulse laser irradiation on the cortex of Paramecium caudatum and Blepharisma undulans undulans were studied. The character and extent of cortical injury was video recorded and subsequently analyzed. Destruction of the cytoskeleton by laser irradiation was detected by immunofluorescence staining. A difference in the development and healing of the wound was observed between Paramecium and Blepharisma cells. A more immediate reaction was recorded in Blepharisma cells containing blepharismin, a red pigment, known to absorb light energy. The damage to the infusorian cortex due to laser irradiation was compared with that produced by mechanical devices.
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